The process of cell culture involves growing and maintaining cells outside of their natural environment. Cell culture media and supplements are critical for supporting the maintenance and growth of cells in vitro, such as by maintaining extracellular pH. Essential components of cell culture media include carbohydrates, amino acids, vitamins, minerals, and a pH buffer system. Your choice of media can affect the accuracy, reproducibility, and relevance of your experimental results. Selecting the right media and supplements is an important step in any experiments that require cell culture.